Sunday, 02 October 2022 16:13

Antibacterial and Antibiofilm Profiles of Lavandula Angustifolia Essential Oil on Clinically Isolated Porphyromonas Gingivalis and Prevotella Intermedia: An In Vitro Study

1Sarwar Ghareeb Husian, BDS and 2Faraedon Mostafa Zardawi, PhD 1Department of Periodontology, College of Dentistry, University of Sulaimani,
Kurdistan Region of Iraq. 2Dean of Faculty of Dentistry, Qaiwan International University, Sulaimani, Kurdistan Region, Iraq. Department of Periodontics, College of Dentistry, University of Sulaimani, Kurdistan Region, Sulaimani, Iraq.
Correspondence: Faraedon Mostafa Zardawi
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract
Background
Gingivitis and periodontitis are common oral diseases. Gingivitis is usually the first stage of periodontal disease. Periodontitis is a bacterial infection of the periodontium that spreads beyond the gingiva.

Objectives This study investigates the antibacterial effect of essential oil extracted from the Lavandula angustifolia (La) against clinically isolated Porphyromonas gingivalis (P. gingivalis) and Prevotella Intermedia (P. intermedia). Materials and Methods Subgingival plaque samples were collected from periodontitis patients with a probing pocket depth of at least 6mm. The essential oil of (La) was extracted using the hydro-distillation method, and GC-MS was performed to determine the essential oil constituent. Clinically isolated bacteria confirmed by anaerobic culture, morphology, gram stain, and PCR (16S rDNA). Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), disc diffusion method, and anti-biofilm activity of the essential oils was evaluated by a qualitative tube method.

Result The antimicrobial effect of (La) essential oil against P. gingivalis was 30μl/mL (MIC and MBC). While the MIC of (La) essential oil against P. intermedia was 7.5μl/mL and MBC were 15μl /mL. The essential oil extracted from (La) revealed comparable antibacterial activity with mean inhibition zones of 12.8±0.76mm and (11.0±0.5mm, 9.4±0.40) for P. gingivalis and P. intermedia, respectively. The anti-biofilm activity of (La) essential oil against P. gingivalis and P. intermedia revealed moderate anti-biofilm activity.

Conclusion (La) essential oil revealed antibacterial efficacy against clinically isolated P. gingivalis and P. intermedia. Hence, it could be applied as an adjunct in periodontal therapy.

 

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