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Monday, 25 March 2024 12:19

Histological Evaluation of Local Application of Equisetum Arvense /Urtica Dioica Extract on Bone Healing in Rabbits Featured

1Suhail Labeeb Hasoon, MSc and 2Ban A. Ghani, PhD
1, 2Department of Oral Diagnosis, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Corresponding author: Suhail Labeeb Hasoon
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Received 20 May, 2023.
Accepted for publication on August 16, 2023.
Published March 20, 2024.

Background Bone repair is a dynamic and intricate process that involves the coordinated actions of many different types of cells. Due to their minimal side effects, the use of medicinal herbs in bone mending has gained growing attention. The anti-inflammatory effects of Equisetum arvense (Eq) and Urtica dioica (UR) extract are utilized in traditional medicine to promote bone repair. Objectives This stUry aimed to evaluate the effect of locally applied Equisetum arvense and Urtica dioica on the bone healing process. Materials and Methods For this experiment, 24 adult male New Zealand rabbits were employed with an average weight between 1.5 and 2.0 kg. Each animal had four holes induced into both femurs, three of which were treated with Eq and UR extract separately and one was treated with a combination of both Eq and UR. The untreated hole served as a control. Scarifications of animals were done after 2 and 4 weeks of healing periods, 12 rabbits for each. Image J software processed Bone specimens for histological and histomorphometric analysis of osteoblasts, osteocytes, osteoclasts, trabecular number, trabecular area, and bone marrow space. Results Histological findings indicated that the bone defects in the Eq and UR showed more bone formation, mineralization, and maturation compared with the control group and the bone defects in combination groups showed more bone formation than that when the extracts were used solely. Multiple group differences for bone cells showed a highly significant difference among all groups in both healing periods. Highly significant results were obtained between both durations regarding trabecular area, trabecular number, and bone marrow area. Conclusions Findings of this stUry revealed that combined local application of (Eq,UR) extracts was more effective in enhancing bone regeneration and accelerating bone healing than the other groups.

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